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KMID : 1132720070050040174
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Genomics & Informatics
2007 Volume.5 No. 4 p.174 ~ p.178
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Conservation of cis-Regulatory Element Controlling Timely Translation in the 3’-UTR of Selected Mammalian Maternal Transcripts
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Lee Hyun-Joo
Lim Yoon-Ki
Chang Samg-Ho
Mim Kwan-Sik
Han Ching-Tack
Hwang Sue-Yun
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Abstract
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The earliest stages of mammalian embryogenesis are governed by the activity of maternally inherited transcripts and proteins. Cytoplasmic polyadenylation of selected maternal mRNA has been reported to be a major control mechanism of delayed translation during preimplantation embryogenesis in mice. The presence of cis-elements required for cytoplasmic polyadenylation (e.g., CPE) can serve as a useful tag in the screening of maternal genes partaking in key functions in the transcriptionally dormant egg and early embryo. However, due to its relative simplicity, UA-rich sequences satisfying the canonical rule of known CPE consensus sequences are often found in the 3’-UTR of maternal transcripts that do not actually undergo cytoplasmic polyadenylation. In this study, we developed a method to confirm the validity of candidate
CPE sequences in a given gene by a multiplex comparison of 3’-UTR sequences between mammalian homologs. We
found that genes undergoing cytoplasmic polyadenylation tend to create a conserved block around the CPE, while
CPE-like sequences in the 3’-UTR of genes lacking cytoplasmic polyadenylation do not exhibit such conservation
between species. Through this cross-species comparison, we also identified an alternative CPE in the 3’-UTR of
tissue-type plasminogen activator (tPA), which is more likely to serve as a functional element. We suggest that
verification of CPEs based on sequence conservation can provide a convenient tool for mass screening of factors
governing the earliest processes of mammalian embryogenesis.
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KEYWORD
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Dcytoplasmic polyadenylation, cytoplasmic polyadenylation element (CPE), delayed translation, maternal transcript, 3’-UTR
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